OrangerV1, Main, Exploration, bibRecord, 002651

In vitro induction, regeneration and analysis of autotetraploids derived from protoplasts and callus treated with colchicine in Citrus

Identifieur interne : 002651 ( Main/Exploration ); précédent : 002650; suivant : 002652

In vitro induction, regeneration and analysis of autotetraploids derived from protoplasts and callus treated with colchicine in Citrus

Auteurs : Shao-Hua Zeng [République populaire de Chine] ; Chuan-Wu Chen [République populaire de Chine] ; LIU HONG [République populaire de Chine] ; Ji-Hong Liu [République populaire de Chine] ; Xiu-Xin Deng [République populaire de Chine]

Source :

Plant cell, tissue and organ culture [ 0167-6857 ] ; 2006.

RBID : Pascal:06-0504710

Descripteurs français

Pascal (Inist)
- In vitro, Induction, Régénération, Protoplaste, Cal, Citrus sinensis, Fortunella, Colchicine, Cycle cellulaire, Chromosome, Cytométrie flux, Végétal.

English descriptors

KwdEn :
- Callus, Cell cycle, Chromosome, Citrus sinensis, Colchicine, Flow cytometry, Fortunella, In vitro, Induction, Protoplast, Regeneration, Vegetals.

Abstract

In the present paper attempts were made to induce chromosome doubling of 'Meiwa' kumquat (Fortunella crassifolia) protoplasts and 'Frost' navel orange (Citrus sinensis Osbeck) embryogenic callus via colchicine treatment. Colchicine decreased protoplast viability, delayed protoplast division and inhibited callus growth, indicating presence of toxicity to cells. Cell lines established from 'Meiwa' protoplasts treated with 0.01 and 0.1% colchicine for 8, 16 and 24 h at each concentration showed different responses when they were cultured on embryoid-induction medium. Flow cytometry (FCM) demonstrated that tetraploids were detected in cell lines and embryoids from all of the treatments, with the highest frequency being 19.23%. As for 'Frost', tetraploid cells were only detected when the callus was treated with 0.1% colchicine for either 4 or 8 days, from which plantlets were regenerated. FCM and chromosome counting confirmed them as true tetraploids. The diploid cells were more active in mitotic division during a 12-day culture and smaller in size than their tetraploid counterpart. Potential applications of the novel tetraploid germplasms obtained through in vitro chromosome doubling to citrus cultivar improvement are discussed.

Affiliations:

République populaire de Chine

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to stream Main, to step Curation: 002651

Le document en format XML

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<front><div type="abstract" xml:lang="en">In the present paper attempts were made to induce chromosome doubling of 'Meiwa' kumquat (Fortunella crassifolia) protoplasts and 'Frost' navel orange (Citrus sinensis Osbeck) embryogenic callus via colchicine treatment. Colchicine decreased protoplast viability, delayed protoplast division and inhibited callus growth, indicating presence of toxicity to cells. Cell lines established from 'Meiwa' protoplasts treated with 0.01 and 0.1% colchicine for 8, 16 and 24 h at each concentration showed different responses when they were cultured on embryoid-induction medium. Flow cytometry (FCM) demonstrated that tetraploids were detected in cell lines and embryoids from all of the treatments, with the highest frequency being 19.23%. As for 'Frost', tetraploid cells were only detected when the callus was treated with 0.1% colchicine for either 4 or 8 days, from which plantlets were regenerated. FCM and chromosome counting confirmed them as true tetraploids. The diploid cells were more active in mitotic division during a 12-day culture and smaller in size than their tetraploid counterpart. Potential applications of the novel tetraploid germplasms obtained through in vitro chromosome doubling to citrus cultivar improvement are discussed.</div>
</front>
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In vitro induction, regeneration and analysis of autotetraploids derived from protoplasts and callus treated with colchicine in Citrus

In vitro induction, regeneration and analysis of autotetraploids derived from protoplasts and callus treated with colchicine in Citrus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri